Sample Collection: A healthcare provider will collect a biological sample, usually sputum, saliva, or sometimes tissue, depending on the suspected site of infection.
DNA Extraction: The DNA is extracted from the collected sample.PCR Amplification: Polymerase Chain Reaction (PCR) is used to amplify any Mycobacterium tuberculosis DNA present in the sample. This process makes it easier to detect the bacteria.
Analysis: The amplified DNA is analyzed to identify the presence of Mycobacterium tuberculosis and its genetic characteristics, including drug resistance